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2×TransTaq-T PCR SuperMix ( +dye)

Ready-to-use mixture of TransTaq-T DNA Polymerase, dNTPs and optimized buffer

2×TransTaq-T PCR SuperMix  ( +dye)

Description

TransTaq-T PCR SuperMix is a ready-to-use mixture of TransTaq-T DNA Polymerase, dNTPs and optimized buffer.

The SuperMix is provided at 2× concentration and used at 1× concentration by adding template, primers and H2O.

Efficiency of PCR products with “A” is equal to EasyTaq DNA polymerase.

It is more suitable for high fidelity TA cloning.

• TransTaq-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq DNA Polymerase
• Extension rate is about 1-2 kb/min
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be cloned into pEASY-T vectors
• Amplification of genomic DNA fragment up to 8 kb

Applications

• Complex templates
• TA cloning

Storage

At -20℃ for two years

Kit Contents

Component

TS/AS122/11

TS/AS122/12

2×TransTaq-T PCR SuperMix(-dye)/(+dye)

1 ml

5×1 ml

ddH2O

1 ml

5 ml

Reaction System

Component

Volume

Final Concentration

Template

Variable

As required

Forward Primer (10 μM)

1 μl

0.2 μM

Reverse Primer (10 μM)

1 μl

0.2 μM

2×TransTaq-T PCR SuperMix

25 μl

ddH2O

Variable

-

Total volume

50 μl

-


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