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2×TransStart® FastPfu PCR SuperMix (-dye )
Description
TransStart FastPfu PCR SuperMix is a ready-to-use mixture of TransStart FastPfu DNA polymerase, dNTPs, and optimized buffer.
The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O.
Applications
Storage
At -20℃ for two years
Kit Contents
Component
TS/AS221/01
TS/AS221/02
2×TransStart FastPfu PCR SuperMix (-dye)
1 ml
5×1 ml
ddH2O
5 ml
Reaction System
Volume
Final Concentration
Template
Variable
As required
Forward Primer (10 μM)
1 μl
0.2 μM
Reverse Primer (10 μM)
2×TransStart FastPfu PCR SuperMix
25 μl
1×
-
Total volume
50 μl