EasyTaq DNA Polymerase
Purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus
Scheda tecnica disponibile solo in lingua inglese.
EasyTaq DNA Polymerase (dNTPs-free)
Description
EasyTaq DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa.
EasyTaq DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity.
EasyTaq DNA Polymerase is suitable for routine amplification.
PCR products are unsuitable for PAGE.
Highlights
- Extension rate is about 1-2 kb/min
- Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned intopEASY®-T vectors
- Amplification of genomic DNA fragment up to 4 kb.
Applications
Unit Definition
One unit of EasyTaq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.
Quality Control
- EasyTaq DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE
- Each batch of EasyTaq DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA
Storage Buffer
20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers.
Storage
Kit Contents
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Component
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TS/AP111/01
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TS/AP111/02
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TS/AP111/03
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TSAP111/04
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EasyTaq DNA Polymerase
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500 U×1
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500 U×6
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2500 U×4
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5000 U×10
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10×EasyTaq Buffer
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1.2 ml×1
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1.2 ml×6
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1.2 ml×20
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1.2 ml×100
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6×DNA Loading Buffer
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1 ml×1
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1 ml×2
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1 ml×4
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1 ml×20
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