TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )
Multiple copy and low copy gene detection
Scheda tecnica disponibile solo in lingua inglese.
TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )
Description
The kit provides all the necessary components for cDNA synthesis from total RNA or mRNA.
It is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds.
The resulting cDNA is suitable for qPCR, not for regular PCR.
Highlights
- Simultaneous genomic DNA removal and cDNA synthesis
- The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA
- qPCR ready cDNA in 15 minutes
- cDNA up to 250 bp
Applications
Multiple copy and low copy gene detection
Storage
At -20℃ for two year
Kit Contents
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Components
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TS/AT341/01 (50 rxns )
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TS/AT341/02 (100 rxns )
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5×TransScript All-in-One SuperMix for qPCR
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200 μl
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400 μl
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5×TransScript All-in-One No-RT Control SuperMix for qPCR
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20 μl
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40 μl
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gDNA Remover
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50 μl
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100 μl
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RNase-free Water
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1 ml
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2×1 ml
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