TransScript First-Strand cDNA Synthesis SuperMix

Provides all the necessary components for cDNA synthesis from total RNA or mRNA

Scheda tecnica disponibile solo in lingua inglese.

Description

TransScript First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by TransScript RT/RI Enzyme Mix and 2xTS Reaction Mix.

Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis
The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 12 kb

Applications

Multiple copy and low copy gene detection

Storage

At -20℃ for two year

Kit Contents

Component	PT/AT301/02	PT/AT301/03
TransScript® RT/RI Enzyme Mix	50 µl	100 µl
2×TS Reaction Mix	500 µl	1 ml
Random Primer (N9) (0.1 µg/µl)	50 µl	100 µl
Anchored Oligo (dT)₁₈ Primer (0.5 µg/µl)	50 µl	100 µl
RNase-free Water	500 µl	1 ml

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