Seleziona la lingua: it-IT en-US
Facebook Twitter Instagram
« Torna indietro | Ti trovi in Ignora collegamenti di navigazione Home » Scienze della vita » Biologia molecolare » Biologia molecolare Linea TS » PCR, RT-PCR, qPCR, qRT-PCR » RT-PCR »   TransScript II First Strand cDNA Synthesis SuperMix

TransScript II First-Strand cDNA Synthesis SuperMix

Provides all the necessary components for cDNA synthesis from total RNA or mRNA

 Scheda tecnica disponibile solo in lingua inglese.

TransScript II First-Strand cDNA Synthesis SuperMix

Description

TransScript II First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA.

The cDNA is efficiently synthesized by TransScript® II RT/RI Enzyme Mix and 2×TS II Reaction Mix.

  • Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis
  • The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR
  • Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA
  • cDNA up to 15 kb

Applications

  • cDNA library construction, 3’ and 5’ RACE
  • Multiple copy and low copy gene detection
  • GC-rich or complex secondary structure RNA template

Storage

At -20℃ for two year

Kit Contents

Component

TS/AH301/02

TS/AH301/03

TransScript II RT/RI Enzyme Mix

50 µl

100 µl

2×TS II Reaction Mix

500 µl

1 ml

Random Primer (N9) (0.1 µg/µl)

50 µl

100 µl

Anchored Oligo(dT)20 Primer (0.5 µg/µl)

50 µl

100 µl

RNase-free Water

500 µl

1 ml


Potrebbero interessarti anche questi articoli
Tutti i diritti sono riservati © 2017 CHEIMIKA SAS DI HEIM JURGENI tuoi acquisti sono garantiti dai principali istituti di credito
Realizzato da NapoliWeb S.r.l. Web Agency Napoli