TransScript II Reverse Transcriptase
Increased thermostability for more full-length cDNA products.
Scheda tecnica disponibile solo in lingua inglese
TransScript II Reverse Transcriptase
Description
TransScript II Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity and increased thermostability.
The enzyme is active at up to 55℃.
It provides higher specificity, higher yield and more full-length cDNA products.
- Increased thermostability for more full-length cDNA products
- Reaction temperature at 42℃-55℃
- Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis
- Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA
- cDNA up to 15 kb
Applications
- First-strand cDNA synthesis, cDNA library construction, 3’ and 5’ RACE
- Multiple copy and low copy gene detection
- GC-rich or complex secondary structure RNA template
Unit Definition
One unit of TransScript® II RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37℃ using Poly(A)/Oligo(dT) as template/primer.
10×TS II RT Buffer
500 mM KCl, 30 mM MgCl2, 100 mM Tris-HCl pH 8.4
Storage
At -20℃ for two year
Kit Contents
|
Component
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TS/AH101/02
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TransScript® II RT
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10.000 U
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10×TS II RT Buffer
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100 μl
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Anchored Oligo(dT)20 Primer (0.5 µg/µl)
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50 μl
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