TransScript-Uni One-Step gDNA Removal and cDNA Synthesis SuperMix

improved version of M-MLV reverse transcriptase with higher thermostability.

Scheda tecnica disponibile solo in lingua inglese.

Description

TransScript-Uni RT is an improved version of M-MLV reverse transcriptase with broad range of reaction temperature(42°C-65°C) and higher thermostability. The suggested reaction temperature is 50°C. The SuperMix contains reagents for simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85°C for 5 seconds.

Highlights

Broad range reaction temperature (42°C -65°C )
Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination
The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR
cDNA up to 20 kb

Applications

Multiple copy and low copy gene detection
GC-rich or complex secondary structure RNA template
cDNA library construction, primer extension, 3’ and 5’ RACE

Storage

At-20°C for two year

Kit Contents

Component	TS/AU311/02	TS/AU311/03
TransScript® -Uni RT/RI Enzyme Mix	50 μl	100 μl
gDNA Remover	50 μl	100 μl
2×TS-Uni Reaction Mix	500 μl	1 ml
Random Primer(N9) (0.1 μg/μl)	50 μl	100 μl
Anchored Oligo(dT)20 Primer (0.5 μg/μl)	50 μl	100 μl
RNase-free Water	500 μl	1 ml

Potrebbero interessarti anche questi articoli

TransTaq DNA Polymerase (HiFi）
TransTaq DNA Polymerase High Fidelity（HiFi）(dNTPs-free) 500 units

€ 363,00
iva esc.

Scopri i dettagli

TransScript First-Strand cDNA Synthesis SuperMix
TransScript First-Strand cDNA Synthesis SuperMix 100 rxns×20 µl Systems

€ 580,50
iva esc.

Scopri i dettagli

TransStart FastPfu Fly DNA Polymerase (with 2.5 mM dNTPs)
TransStart FastPfu Fly DNA Polymerase (with 2.5 mM dNTPs) 500 units

€ 874,50
iva esc.

Scopri i dettagli

2×TransStart KD Plus PCR SuperMix
2×TransStart KD Plus PCR SuperMix 5×1 ml

€ 1.140,00
iva esc.

Scopri i dettagli