TransStart KD Plus DNA Polymerase (with 2.5 mM dNTPs)
Genetically modified high fidelity DNA polymerase
Scheda tecnica disponibile solo in lingua inglese.
TransStart KD Plus DNA Polymerase (with 2.5 mM dNTPs)
Description
TransStart KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase.
This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3’-5’ exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
Highlights
• PCR products can be directly cloned into pEASY-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.
Applications
• Fast, high specificity amplification
• High fidelity, high yield amplification
Storage Buffer
50 mM Tris-Cl (pH 8.0)，50 mM KCl，1 mM DTT，0.1 mM EDTA，Stabilizers，50% glycerol.
Storage
At -20 ℃ for two years
Kit Contents
|
Component
|
TS/AP301/11
|
TS/AP301/12
|
TS/AP301/13
|
|
TransStart KD Plus DNA Polymerase
|
100 U×1
|
200 U×1
|
200 U×6
|
|
5×TransStart KD Plus Buffer
|
1.2 ml×1
|
1.2 ml ×2
|
1.2 ml ×12
|
|
2.5 mM dNTPs
|
- / 500 µl×1
|
- / 1 ml ×1
|
- / 1 ml ×6
|
|
50 mM MgSO4
|
200 µl×1
|
400 µl ×1
|
1 ml ×1
|
|
6×DNA Loading Buffer
|
500 μl×1
|
1 ml ×1
|
1 ml ×2
|