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2×EasyTaq PCR SuperMix for PAGE (+dye)

Mixture of EasyTaq® DNA Polymerase for PAGE, dNTPs and optimized buffer

2×EasyTaq PCR SuperMix for PAGE (+dye)

Description

EasyTaq PCR SuperMix for PAGE is a ready-to-use mixture of EasyTaq DNA Polymerase for PAGE, dNTPs and optimized buffer.

The SuperMix for PAGE is provided at 2× concentration and used at 1× concentration by adding template, primers and H2O.

  • Extension rate is about 1-2 kb/min
  • Unique buffer system compatible with PAGE
  • Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be cloned into pEASY®-T vectors
  • Amplification of genomic DNA fragment up to 3 kb

Application

Short fragment PCR

Storage

At -20℃ for two years

Kit Contents

Component

TS/AS112/11

TS/AS112/12

TS/AS112/13

EasyTaq PCR SuperMix for PAGE (+dye)

1 ml

5×1 ml

15×1 ml

ddH2O

1 ml

5 ml

3×5 ml

Reaction System

Component

Volume

Final Concentration

Template

Variable

As required

Forward Primer (10 μM)

1 μl

0.2 μM

Reverse Primer (10 μM)

1 μl

0.2 μM

EasyTaq PCR SuperMix for PAGE

25 μl

ddH2O

Variable

-

Total volume

50 μl

-


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