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2×TransStart FastPfu PCR SuperMix (-dye )

Ready-to-use mixture of TransStart® FastPfu DNA polymerase, dNTPs, and optimized buffer

2×TransStart® FastPfu PCR SuperMix (-dye )

Description

TransStart FastPfu PCR SuperMix is a ready-to-use mixture of TransStart FastPfu DNA polymerase, dNTPs, and optimized buffer.

The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O.

  • TransStart FastPfu PCR SuperMix offers 54-fold fidelity as compared to EasyTaq DNA Polymerase
  • Extension rate is about 2-4 kb/min
  • PCR products can be directly cloned into pEASY-Blunt vectors
  • Amplification of genomic DNA fragment up to 15 kb
  • Amplification of plasmid DNA fragment up to 20 kb

Applications

  • High fidelity PCR
  • High yield PCR
  • Fast PCR
  • Blunt end cloning
  • Site-directed mutagenesis
  • Complex templates

Storage

At -20℃ for two years

Kit Contents

Component

TS/AS221/01

TS/AS221/02

2×TransStart FastPfu PCR SuperMix (-dye)

1 ml

5×1 ml

ddH2O

1 ml

5 ml

Reaction System

Component

Volume

Final Concentration

Template

Variable

As required

Forward Primer (10 μM)

1 μl

0.2 μM

Reverse Primer (10 μM)

1 μl

0.2 μM

2×TransStart FastPfu PCR SuperMix

25 μl

ddH2O

Variable

-

Total volume

50 μl

-


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