Adenovirus Purification Kit
Fast adenovirus purification method with high recovery
This Adenovirus purification kit is a fast adenovirus purification method with high recovery.
The adenovirus can be purified in 60 minutes while the traditional CsCl ultracentrifugation takes 24 hours.
The Kit provides 5 columns 10 preparations and each preparation can produce up to 3 x 1012 Vps.
The purified virus can be used for infection of cells and animals.
Features
The Adenovirus maxi purification kit designed for fast and efficient purification of recombinant Adenovirus from adenovirus transfected cell culture supernatant.
Up to 4 x1012 viral particles can be purified from cell culture media of 6 to 8 T75 flasks.
Traditionally the recombinant lentivirus is purified by ultra centrifugation using CsCl to separate the virus particles from cellular proteins and media components. The CsCl ultracentrifugation procedure is time consuming and limited to the amount of cell lysate to be processed.
Each column can be regenerated for purifying the same adenovirus. For optimized viral binding and recovery, each column can be regenerated only once.
Storage and Stability
All components are guaranteed for at least 12 months from the date of purchase when stored as follows: Mini column and desalting tube should be stored at 4 °C, and all other materials at RT (22-25°C).
Kit contents
| Code |
V1260-00 |
V1260-01 |
V1260-02 |
Notes |
| Preps |
2 |
4 |
10 |
|
| Maxi Columns |
1 |
2 |
5 |
Can be used twice
(Store at 4°C) |
| Press-On Cap |
1 |
2 |
5 |
Store at RT |
| Desalting Tube* |
Not Included |
1 |
1 |
Can be regenarated
(Store at 4°C) |
| 50 mL Collection Tube |
2 |
4 |
10 |
Store at RT |
| 10 x Wash Buffer |
6 ml |
30 ml |
60 ml |
Store at RT |
| 2 x Elution Buffer |
6 ml |
30 ml |
60 ml |
Store at RT |
| Regeneration Buffer |
6 ml |
30 ml |
60 ml |
Store at RT |
*Desalting tube could be regenarated and applied on one kind of virus. Desalting tube could be purchased from Biomiga.
Safety Consideration
The adenovirus infected cell media and the purified virus can be potential bio-hazardous material and can be infectious to human and animals. All protocols MUST be performed under at least Bio-Safety level 2 working condition.
Materials required but not supplied
- ddH2O
- PBS
- 0.45 µm and 0.22 µm filters
- Rack holder for columns