BAT BROTH - 500 g
For the detection and enrichment of Alicyclobacillus in fruit juices and other acid foods, according method nº 12 of IFU norm
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Suspend 11 grams of the medium in one liter of distilled water .Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes. Cool to 45-50ºC and adjust the pH at 4.0 ± 0.2 after autoclaving, adding sufficient quantity of 1 N H2SO4. Avoid overheating after adjusting the pH.
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