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BLOOD AGAR BASE

For the isolation, cultivation and detection of hemolytic activity of fastidious microorganisms

BLOOD AGAR BASE - 500 g

For the isolation, cultivation and detection of hemolytic activity of fastidious microorganisms

Applications

  • Hemolytic Activity
  • Isolation
  • AEROMONAS
  • OTHER APPLICATIONS

Packaging

Suspend 40 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add 5-10% of sterile defibrinated blood, homogenize and pour intro Petri dishes. Be careful to avoid bubble formation when adding the blood to the cooled medium and rotate the flask or bottle slowly to create a homogeneous solution. If desired, Polyenrichment Supplement (Cat. 6011) may be added to increase growth.

Formula

  • Heart Infusion 10
  • Meat Peptone 10
  • Sodium Chloride 5
  • Bacteriological Agar 15
Recommended supplement (to be purchased separately)
  • POLYENRICHMENT Supplement-selective supplement for the isolation of Neisseria and Haemophilus

 


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