CARBOHYDRATES UTILIZATION BROTH BASE (ISO 11290) - 500 g
For the confirmation of Listeria monocytogenes based on carbohydrates utilization
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Suspend 16.02 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add 1 ml of a filtered 5% solution of L-Ramnose or D-Xylose.
Formula