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DESOXYCHOLATE LACTOSE AGAR

Differential and slightly selective medium for the isolation of Gram negative enteric bacilli

DESOXYCHOLATE LACTOSE AGAR - 500 g

Differential and slightly selective medium for the isolation of Gram negative enteric bacilli

Applications

  • Isolation
  • Selective isolation
  • COLIFORMS
  • SALMONELLA

Packaging

Suspend 42.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. AVOID OVERHEATING. DO NOT AUTOCLAVE. Cool to 45-50ºC and dispense into Petri dishes. Prepared plates may present a slight precipitate. NOTE: Overheating may increase the degree of inhibition.

Formula

  • Bacteriological Peptone 10
  • Lactose 10
  • Sodium Chloride 5
  • Sodium Citrate 2
  • Sodium Desoxycholate 0.5
  • Neutral Red 0.03
  • Bacteriological Agar 15

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