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EVA BROTH (ETHYL VIOLET AZIDE)

Selective medium for the confirmation and detection of Enterococci

EVA BROTH (ETHYL VIOLET AZIDE, LITSKY) - 500 g

Selective medium for the confirmation and detection of Enterococci and as a detector of fecal contamination in water

Applications

  • Detection / Isolation
    ENTEROCOCCUS

Packaging

Suspend 35.8 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense 10 ml amounts into test tubes and sterilize in autoclave at 121°C for 15 minutes. It is recommended to use a large inoculum as the medium is very selective and is used in the second phase of confirmation.

Caution: This medium is toxic if swallowed, inhaled or comes in contact with skin. Wear gloves and eye/face protection.

Formula

  • Peptone Mixture 20
  • Dextrose 5
  • Sodium Chloride 5
  • Monopotassium Phosphate 2.7
  • Dipotassium Phosphate 2.7
  • Sodium Azide 0.4
  • Ethyl Violet 0.0008

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