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EasyScript Reverse Transcriptase

Engineered version of M-MLV reverse transcriptase with deficient RNase H activity

EasyScript  Reverse Transcriptase

EasyScript Reverse Transcriptase is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity.

The enzyme is purified to near homogeneity from E.coli containing the modified M-MLV RT gene.

  • Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis
  • Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA
  • cDNA up to 8 kb

Applications

  • First-strand cDNA synthesis
  • Multiple copy gene detection

Unit Definition

One unit of EasyScript RT incorporates 1 nmol of deoxyribonucleotide into acid precipitable material in 10 minutes at 37℃ using Poly(A)/Oligo(dT) as template/primer.

5×ES RT Buffer

375 mM KCl, 15 mM MgCl2, 100 mM Tris-HCl pH 8.4


Storage

At -20℃ for two year

Kit contents

Component

TS/AE101/02

TS/AE101/03

EasyScript RT

10.000 U

5×10.000 U

5×ES RT Buffer

200 μl

5×200 μl

Anchored Oligo(dT)18 Primer (0.5 µg/µl)

50 μl

250 μl


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