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EasyTaq DNA Polymerase

Purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus

 Scheda tecnica disponibile solo in lingua inglese.

EasyTaq DNA Polymerase (dNTPs-free)


Description

EasyTaq DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa.

EasyTaq  DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity.

EasyTaq DNA Polymerase is suitable for routine amplification.

PCR products are unsuitable for PAGE.


Highlights

  • Extension rate is about 1-2 kb/min
  • Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned intopEASY®-T vectors
  •  Amplification of genomic DNA fragment up to 4 kb.


Applications

  • Routine PCR
  • Colony PCR


Unit Definition

One unit of EasyTaq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.


Quality Control

  • EasyTaq DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE
  • Each batch of EasyTaq DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA


Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers.


Storage

  • At -20°C for two years

Kit Contents

Component

TS/AP111/01 

TS/AP111/02 

TS/AP111/03 

TSAP111/04

EasyTaq DNA Polymerase

500 U×1

500 U×6

2500 U×4

5000 U×10

10×EasyTaq Buffer

1.2 ml×1

1.2 ml×6

1.2 ml×20

1.2 ml×100

6×DNA Loading Buffer

1 ml×1

1 ml×2

1 ml×4

1 ml×20


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