EasyTaq DNA Polymerase for PAGE (with 2.5 mM dNTPs)
Purified from E. coliexpressing a cloned DNA polymerase from Thermus aquaticus
Scheda tecnica disponibile solo in lingua inglese.
EasyTaq DNA Polymerase for PAGE (with 2.5 mM dNTPs)
Description
EasyTaq DNA Polymerase for PAGE is purified from E. coliexpressing a cloned DNA polymerase from Thermus aquaticus.
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa.
EasyTaq DNA Polymerase for PAGE has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. lt lacks 3′-5′ exonuclease activity.
This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.
Highlights
- Extension rate is about 1-2 kb/min
- Unique buffer system compatible with PAGE
- Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned intopEASY-T vectors
- Amplification of genomic DNA fragment up to 3 kb
Applications
Short fragment PCR
Unit Definition
One unit of EasyTaq DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.
Quality Control
EasyTaq DNA Polymerase for PAGE has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE.
Each batch of EasyTaq DNA Polymerase for PAGE has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.
Storage Buffer
20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers
Storage
Kit Contents
|
Component
|
TS/AP112/11
|
TS/AP112/12
|
|
EasyTaq® DNA Polymerase for PAGE
|
2500 U
|
2500 U×4
|
|
10×EasyTaq® Buffer for PAGE
|
1.2 ml ×5
|
1.2 ml×20
|
|
2.5 mM dNTPs
|
- / 800 µl×5
|
- / 800 µl×20
|
|
6×DNA Loading Buffer
|
1 ml
|
1ml ×4
|