LACTOSE SULFITE BROTH BASE EUROPEAN PHARMACOPOEIA ISO 7937 - 500 g
Base medium used for search for Clostridium perfringens ISO 7937
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Suspend 20.3 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into tubes with Durham gas collecting tubes for gas detection. Sterilize in autoclave at 121°C for 15 minutes. Store at 4ºC. Before using add to each tube 0.5 ml of a 12 g/liter solution of Sodium Metabisulfite and 0.5 ml of a solution of 10 g/liter of Ferric Ammonium Citrate. Both solutions have to be freshly prepared and sterilized.
Formula