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TransFast Taq DNA Polymerase (with 2.5 mM dNTPs)

An engineered version of Taq DNA Polymerase

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TransFastTaq DNA Polymerase (with 2.5 mM dNTPs)

Description

TransFast Taq DNA Polymerase is an engineered version of Taq DNA Polymerase.

The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa.

TransFast Taq DNA Polymerase has 5’-3’DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’exonuclease activity.


Highlights

  • Extension rate is about 6 kb/min
  • Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY-T vectors
  • Amplification of genomic DNA fragment up to 4 kb


Applications

  • Routine PCR
  • High throughput PCR
  • Colony PCR


Unit Definition

  • One unit of TransFast Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃


Quality Control

TransFast Taq DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE.

Each batch of TransFast Taq DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.


Storage Buffer

  • 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers


Storage

  • At -20 ℃ for two years

Kit Contents

Component

TS/AP101/11

TS/AP101/12

TransFast® Taq DNA Polymerase

500 units

6×500 units

10× TransFast® Taq Buffer

1.2ml x 1

1.2ml x 6

2.5mM dNTPs

- / 800 μl x 1

- / 800 μl x 6

6×DNA Loading Buffer

1ml x 1

1ml x 6


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