TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )
Description
The kit provides all the necessary components for cDNA synthesis from total RNA or mRNA.
It is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds.
The resulting cDNA is suitable for qPCR, not for regular PCR.
Highlights
Applications
Multiple copy and low copy gene detection
Storage
At -20℃ for two year
Kit Contents
Components
TS/AT341/01 (50 rxns )
TS/AT341/02 (100 rxns )
5×TransScript All-in-One SuperMix for qPCR
200 μl
400 μl
5×TransScript All-in-One No-RT Control SuperMix for qPCR
20 μl
40 μl
gDNA Remover
50 μl
100 μl
RNase-free Water
1 ml
2×1 ml