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TransScript Fly First-Strand cDNA Synthesis SuperMix

Provides high affinity to RNA template with fast extension rate

TransScript Fly First-Strand cDNA Synthesis SuperMix

Description

TransScript Fly RT is generated by site mutations. It provides high affinity to RNA template with fast extension rate.

The cDNA is efficiently synthesized by TransScript Fly RT/RI Enzyme Mix and 2×TS Fly Reaction Mix.

The entire reverse transcription can be completed within 5 minutes.


Highlights

  • 5 minutes reverse transcription
  • Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis
  • Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA
  • cDNA up to 12 kb


Applications

Multiple copy and low copy gene fast detection


Storage

At -20℃ for two year


Kit Contents

Component

TS/AF301/02

TS/AF301/03

TransScript® Fly RT/RI Enzyme Mix

50 µl

100 µl

2×TS Fly Reaction Mix

500 µl

1 ml

Random Primer(N9) (0.1 µg/µl)

50 µl

100 µl

Anchored Oligo(dT)18 Primer (0.5 µg/µl)

50 µl

100 µl

RNase-free Water

500 µl

1 ml


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