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TransScript II All-in-One First-Strand cDNA Synthesis SuperMix for PCR

Provides all the necessary components for cDNA synthesis from total RNA or mRNA

TransScript II All-in-One First-Strand cDNA Synthesis SuperMix for PCR

Description

TransScript II All-in-One First-Strand cDNA Synthesis SuperMix for PCR provides all the necessary components for cDNA synthesis from total RNA or mRNA.

The SuperMix is provided at 5× concentration and used at 1× concentration by adding RNA and H2O. The resulting cDNA is suitable for regular PCR, not for qPCR.

Highlights

  • One-tube format for simple and fast setup and reduced pipetting variability
  • The optimal ratio of oligo(dT)20 primer to random primer(N9) for PCR ready cDNA
  • PCR ready cDNA in 30 minutes (unsuitable for qPCR)
  • cDNA up to 15 kb

Applications

  • cDNA library construction, 3’ and 5’ RACE
  • Multiple copy and low copy gene detection
  • GC-rich or complex secondary structure RNA template

Storage

At -20℃ for two year

Kit Contents

Components

TS/AH321/01 (50 rxns )

5×TransScript II All-in-One SuperMix for PCR

200 μl

RNase-free Water

1 ml

 


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