TransScript Reverse Transcriptase

Recombinant M-MLV reverse transcriptase with deficient RNase H activity.

Description

TransScript Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity.

Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA
cDNA up to 12 kb

Applications

First-strand cDNA synthesis
Multiple copy and low copy gene detection

Unit Definition

One unit of TransScript RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37℃ using Poly(A)/Oligo(dT) as template/primer.

5×TS RT Buffer

250 mM KCl, 15 mM MgCl₂, 100 mM Tris-HCl pH 8.4

Storage

At -20℃ for two year

Kit Contents

Component	TS/AT101/02	TS/AT101/03
TransScript RT	10000 U	5×10000 U
5×TS RT Buffer	200 μl	5×200 μl
Anchored Oligo(dT)18 Primer (0.5 µg/µl)	50 μl	250 μl

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