TransStart FastPfu Fly DNA Polymerase (with 2.5 mM dNTPs)
Hot start, high fidelity and high processivity DNA Polymerase
TransStart FastPfu Fly DNA Polymerase - (with 2.5 mM dNTPs)
Description
TransStart FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase.
TransStart FastPfuFly DNA Polymerase has an extension rate of up to 6 kb/min.
Compared with TransStart FastPfu DNA Polymerase, TransStart®FastPfu Fly DNA Polymerase has higher extension rate, higher fidelity, and higher amplification efficiency.
Highlights
• TransStart FastPfu Fly DNA Polymerase offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase
• Extension rate is about 2-6 kb/min
• PCR products can be directly cloned into pEASY®-Blunt vectors
• Amplification of genomic DNA fragment up to 15 kb
• Amplification of plasmid DNA fragment up to 20 kb
Applications
• High fidelity PCR
• High yield and fast PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates
Unit Definition
One unit of TransStart FastPfu Fly DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.
Quality Control
TransStart FastPfu Fly DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE.
Each batch of TransStart FastPfu Fly DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.
Storage Buffer
50 mM Tris-HCl (pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50% glycerol
Storage
Kit Contents
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Component
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TS/AP231/11
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TS/AP231/12
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TS/AP231/13
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TransStart FastPfu Fly DNA Polymerase
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250 U×1
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500 U×1
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500 U×6
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5×TransStart FastPfu Fly Buffer
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1.2 ml×1
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1.2 ml ×2
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1.2 ml ×12
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2.5 mM dNTPs
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- / 500 μl×1
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- / 1 ml ×1
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- / 1 ml ×6
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50 mM MgSO4
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200 µl×1
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400 µl ×1
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1 ml ×1
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PCR Stimulant
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200 µl×1
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400 µl×1
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1 ml ×1
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6×DNA Loading Buffer
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500 μl×1
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1 ml ×1
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1 ml ×2
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