TransStart TopTaq DNA Polymerase (with 2.5 mM dNTPs)
Engineered version of Taq DNA Polymerase combined with TransStart technique
TransStart TopTaq DNA Polymerase (with 2.5 mM dNTPs) DNA POLIMERASI
Description
TransStart TopTaq DNA Polymerase is an engineered version of Taq DNA Polymerase combined with TransStart technique.
One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature.
Other two binding proteins bind primers, preventing primer-dimer formation.
Blocking proteins are released from primers and templates during the initial denaturation.
This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Highlights
• Compared with TransStart Taq DNA Polymerase, TransStart®TopTaq DNA Polymerase has higher amplification efficiency, specificity and sensitivity
• TransStart TopTaq DNA Polymerase offers 18-fold fidelity as compared toEasyTaq® DNA Polymerase
• The specificity is higher than antibody based or chemically modified hot start DNA polymerases
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors
• Reduced nonspecific amplification and primer dimer formation
• Different from Taq antibody, no risk of contamination from mammalian DNA
• Different from chemical modification, long denaturing step is not needed
• Amplification of genomic DNA fragment up to 15 kb
Applications
• Complex templates
• GC/AT-rich templates
• Multiplex PCR
• High yield PCR
Unit Definition
One unit of TransStart TopTaq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.
Quality Control
TransStart TopTaq DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE.
Each batch of TransStartTopTaq DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.
Storage Buffer
20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers.
Storage
Kit Contents
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Component
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TS/AP151/11
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TS/AP151/12
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TS/AP151/13
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TransStart TopTaq DNA Polymerase
|
250 U×1
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500 U×1
|
500 U×6
|
|
10×TransStart TopTaq Buffer
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1.2 ml
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1.2 ml×2
|
1.2 ml×12
|
|
2.5 mM dNTPs
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-/800 μl×1
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-/800 μl×2
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-/1.2 ml×8
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|
10×GC Enhancer
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200 μl×1
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400 μl×1
|
1 ml×1
|
|
6×DNA Loading Buffer
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500 μl×1
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1 ml×1
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1 ml×2
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