TransTaq DNA Polymerase (HiFi）(with 2.5 mM dNTPs)
Provides higher specificity and higher amplification efficiency
TransTaq DNA Polymerase High Fidelity （HiFi) - (with 2.5 mM dNTPs)
Description
TransTaq DNA Polymerase High Fidelity (TransTaqHiFi DNA Polymerase) contains TransTaq-T DNA Polymerase and a proofreading 3’-5’ exonuclease.
TransTaq HiFi DNA Polymerase provides higher specificity and higher amplification efficiency thanTransTaq-T DNA Polymerase.
Two different buffers are provided in the kit.TransTaq HiFi Buffer I is optimized for the amplification of genomic DNA andTransTaq HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
Highlights
- TransTaq HiFi DNA Polymerase offers 18-fold fidelity as compared toEasyTaq DNA Polymerase
- Extension rate is about 1-2 kb/min
- Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned intopEASY-T vectors
- Amplification of genomic DNA fragment up to 15 kb
Applications
- Complex templates
- GC/AT rich templates
- Long PCR
- High yield PCR
Unit Definition
One unit of TransTaq HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.
Quality Control
TransTaq HiFi DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE.
Each batch ofTransTaq HiFi DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.
Storage Buffer
20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers.
Storage
At -20 ℃ for two years
Kit Contents
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Component
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TS/AP131/11
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TS/AP131/12
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TS/AP131/13
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TransTaq® HiFi DNA Polymerase
|
250 U×1
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500 U×1
|
500 U×6
|
|
10×TransTaq® HiFi Buffer I
|
1.2 ml×1
|
1.2 ml×1
|
1.2 ml×6
|
|
10×TransTaq® HiFi Buffer II
|
1.2 ml×1
|
1.2 ml×1
|
1.2 ml×6
|
|
2.5 mM dNTP
|
-/400 μl×1
|
-/800 μl×1
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-/800 μl×6
|
|
10×GC Enhancer
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200 μl×1
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400 μl×1
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1 ml×1
|
|
6×DNA Loading Buffer
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500 μl×1
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1 ml×1
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1 ml×2
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