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EasyPfu DNA Polymerase

Engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity

EasyPfu DNA Polymerase ( dNTPs-FREE)

Description

EasyPfu DNA Polymerase is an engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity. EasyPfu DNAPolymerase possesses a proofreading 3'-5' exonuclease activity.


Highlights

• EasyPfu DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 0.5 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 6 kb.
• Amplification of plasmid DNA fragment up to 10 kb.


Applications

• High fidelity PCR
• Blunt-end cloning
• Site-directed mutagenesis


Unit Definition

One unit of EasyPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.


Quality Control

EasyPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE.

Each batch of EasyPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.


Storage Buffer

50 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% (v/v) glycerol stabilizers

Storage

At -20°C for two years


Kit Contents

Component

TS/AP211/01

TS/AP211/02

TS/AP211/03

EasyPfu DNA Polymerase

250 U×1

500 U×1

500 U×6

10×EasyPfu Buffer

1.2 ml ×1

1.2 ml ×1

1.2 ml ×6

6×DNA Loading Buffer

500 μl×1

1 ml ×1

1 ml ×2

50 mM MgSO4

200 μl×1

400 μl×1

1 ml ×1


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