EasyPfu DNA Polymerase
Engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity
EasyPfu DNA Polymerase ( dNTPs-FREE)
Description
EasyPfu DNA Polymerase is an engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity. EasyPfu DNAPolymerase possesses a proofreading 3'-5' exonuclease activity.
Highlights
• EasyPfu DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 0.5 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 6 kb.
• Amplification of plasmid DNA fragment up to 10 kb.
Applications
• High fidelity PCR
• Blunt-end cloning
• Site-directed mutagenesis
Unit Definition
One unit of EasyPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.
Quality Control
EasyPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE.
Each batch of EasyPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.
Storage Buffer
50 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% (v/v) glycerol stabilizers
Storage
At -20°C for two years
Kit Contents
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Component
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TS/AP211/01
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TS/AP211/02
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TS/AP211/03
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EasyPfu DNA Polymerase
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250 U×1
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500 U×1
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500 U×6
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10×EasyPfu Buffer
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1.2 ml ×1
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1.2 ml ×1
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1.2 ml ×6
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6×DNA Loading Buffer
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500 μl×1
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1 ml ×1
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1 ml ×2
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50 mM MgSO4
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200 μl×1
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400 μl×1
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1 ml ×1
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