EasyPfu DNA Polymerase (with 2.5 mM dNTPs)
Engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity
EasyPfu DNA Polymerase (with 2.5 mM dNTPs)
Description
EasyPfu DNA Polymerase is an engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity. EasyPfu DNAPolymerase possesses a proofreading 3'-5' exonuclease activity.
Highlights
• EasyPfu DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 0.5 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 6 kb.
• Amplification of plasmid DNA fragment up to 10 kb.
Applications
• High fidelity PCR
• Blunt-end cloning
• Site-directed mutagenesis
Unit Definition
One unit of EasyPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.
Quality Control
EasyPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE.
Each batch of EasyPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.
Storage Buffer
50 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% (v/v) glycerol stabilizers
Storage
At -20°C for two years
Kit Contents
|
Component
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TS/AP211/11
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TS/AP211/12
|
TS/AP211/13
|
|
EasyPfu DNA Polymerase
|
250 U×1
|
500 U×1
|
500 U×6
|
|
10×EasyPfu Buffer
|
1.2 ml ×1
|
1.2 ml ×1
|
1.2 ml ×6
|
|
2.5 mM dNTPs
|
- / 500 µl ×1
|
- / 1 ml ×1
|
- / 1 ml ×6
|
|
6×DNA Loading Buffer
|
500 μl×1
|
1 ml ×1
|
1 ml ×2
|
|
50 mM MgSO4
|
200 μl×1
|
400 μl×1
|
1 ml ×1
|