TransStart FastPfu DNA Polymerase
Fast, high fidelity and high processivity hot start DNA polymerase
TransStart FastPfu DNA Polymerase
Description
TransStart FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.
Highlights
• Extension rate is about 2-4 kb/min
• TransStart® FastPfu DNA Polymerase offers 54-fold fidelity as compared to EasyTaq®DNA Polymerase
• PCR products can be directly cloned into pEASY®-Blunt vectors
• Amplification of genomic DNA fragment up to 15 kb
• Amplification of plasmid DNA fragment up to 20 kb
Applications
• High fidelity PCR
• High yield and fast PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates
Unit Definition
One unit of TransStart FastPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.
Quality Control
TransStart FastPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE.
Each batch of TransStart FastPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.
Storage Buffer
50 mM Tris-HCl (pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50% glycerol
Storage
At -20 ℃ for two years
Kit Contents
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Component
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TS/AP221/01
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TS/AP221/02
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TS/AP221/03
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TransStart® FastPfu DNA Polymerase
|
250 U×1
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500 U×1
|
500 U×6
|
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5×TransStart FastPfu Buffer
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1.2 ml×1
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1.2 ml ×2
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1.2 ml ×12
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2.5 mM dNTPs
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- / 500 µl×1
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- / 1 ml ×1
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- / 1 ml ×6
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50 mM MgSO4
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200 µl×1
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400 µl ×1
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1 ml ×1
|
|
PCR Stimulant
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200 µl×1
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400 µl×1
|
1 ml ×1
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6×DNA Loading Buffer
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500 μl×1
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1 ml ×1
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1 ml ×2
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