TransTaq-T DNA Polymerase (with 2.5 mM dNTPs)
Mixture of EasyTaq DNA Polymerase with a proofreading 3’-5’ exonuclease
Scheda tecnica disponibile solo in lingua inglese.
TransTaq-T DNA Polymerase (with 2.5 mM dNTPs)
Description
TransTaq-T DNA Polymerase is a mixture of EasyTaq DNA Polymerase with a proofreading 3’-5’ exonuclease.
The fidelity is equal to EasyPfu DNA Polymerase.
The yield is equal to that fromEasyTaq DNA Polymerase. It is more suitable for high fidelity TA cloning.
Highlights
- TransTaq-T DNA Polymerase offers 18-fold fidelity as compared to EasyTaq DNA Polymerase
- Extension rate is about 1-2 kb/min
- Template-independent“A”can be generated at the 3’end of the PCR product
- PCR products can be directly cloned intopEASY®-T vectors
- Amplification of genomic DNA fragment up to 8 kb
Applications
- Complex templates
- TA cloning
Unit Definition
One unit of TransTaq-T DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.
Quality Control
TransTaq-T DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE.
Each batch of TransTaq-T DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.
Storage Buffer
20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers
Storage
Kit Contents
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Component
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TS/AP122/11
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TS/AP122/12
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TS/AP122/13
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TransTaq-T DNA Polymerase
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250 U×1
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500 U×1
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500 U×6
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10×TransTaq-T Buffer
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1.2 ml ×1
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1.2 ml ×1
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1.2 ml ×6
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2.5 mM dNTPs
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- / 400 µl ×1
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- / 800 µl ×1
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- / 800 µl ×6
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6×DNA Loading Buffer
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500 μl×1
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1 ml ×1
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1 ml ×2
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