TransStart FastPfu DNA Polymerase (with 2.5 mM dNTPs)
Fast, high fidelity and high processivity hot start DNA polymerase
TransStart FastPfu DNA Polymerase (with 2.5 mM dNTPs)
Description
TransStart FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.
Highlights
• Extension rate is about 2-4 kb/min
• TransStart® FastPfu DNA Polymerase offers 54-fold fidelity as compared to EasyTaq®DNA Polymerase
• PCR products can be directly cloned into pEASY®-Blunt vectors
• Amplification of genomic DNA fragment up to 15 kb
• Amplification of plasmid DNA fragment up to 20 kb
Applications
• High fidelity PCR
• High yield and fast PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates
Unit Definition
One unit of TransStart FastPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.
Quality Control
TransStart FastPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE.
Each batch of TransStart FastPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.
Storage Buffer
50 mM Tris-HCl (pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50% glycerol
Storage
At -20 ℃ for two years
Kit Contents
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Component
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TS/AP221/11
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TS/AP221/12
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TS/AP221/13
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TransStart® FastPfu DNA Polymerase
|
250 U×1
|
500 U×1
|
500 U×6
|
|
5×TransStart FastPfu Buffer
|
1.2 ml×1
|
1.2 ml ×2
|
1.2 ml ×12
|
|
2.5 mM dNTPs
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- / 500 µl×1
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- / 1 ml ×1
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- / 1 ml ×6
|
|
50 mM MgSO4
|
200 µl×1
|
400 µl ×1
|
1 ml ×1
|
|
PCR Stimulant
|
200 µl×1
|
400 µl×1
|
1 ml ×1
|
|
6×DNA Loading Buffer
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500 μl×1
|
1 ml ×1
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1 ml ×2
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