EasyTaq DNA Polymerase for PAGE
Purified from E. coliexpressing a cloned DNA polymerase from Thermus aquaticus
EasyTaq DNA Polymerase for PAGE (dNTPs-free)
Description
EasyTaq DNA Polymerase for PAGE is purified from E. coliexpressing a cloned DNA polymerase from Thermus aquaticus.
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa.
EasyTaq DNA Polymerase for PAGE has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. lt lacks 3′-5′ exonuclease activity.
This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.
Highlights
- Extension rate is about 1-2 kb/min
- Unique buffer system compatible with PAGE
- Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned intopEASY-T vectors
- Amplification of genomic DNA fragment up to 3 kb
Applications
Short fragment PCR
Unit Definition
One unit of EasyTaq DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.
Quality Control
EasyTaq DNA Polymerase for PAGE has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE.
Each batch of EasyTaq DNA Polymerase for PAGE has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.
Storage Buffer
20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers
Storage
Kit Contents
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Component
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TS/AP112/01
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TS/AP112/02
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EasyTaq® DNA Polymerase for PAGE
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2500 U
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2500 U×4
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10×EasyTaq® Buffer for PAGE
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1.2 ml ×5
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1.2 ml×20
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6×DNA Loading Buffer
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1 ml
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1ml ×4
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