CAMPYLOBACTER AGAR BASE
Selective medium for Campylobacter jejuni and C.coli
CAMPYLOBACTER AGAR BASE (PRESTON) - 500 g
Selective medium for Campylobacter jejuni and C.coli
Applications
- Selective isolation
CAMPYLOBACTER
Packaging
Suspend 18.5 grams of the medium in 475 ml of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45ºC and aseptically add 5-7% of lysed horse blood and one vial of Preston Campylobacter Supplement (Cat. 6019), previously reconstituted with 5 ml of 1:1 solution of acetone / sterile distilled water. Homogenize gently and dispense into Petri dishes. Be careful to avoid bubble formation when adding the blood to the cooled medium, and rotate the flask or bottle slowly to create a homogeneous solution. The prepared medium should be stored at 8-15°C. The color of the prepared medium is yellowish white. The color of the prepared medium with blood is cherry red opaque.
Formula
- Casein Peptone 10
- Beef Extract 10
- Sodium Chloride 5
- Bacteriological Agar 12
Recommended supplement (to be purchased separately)
- PRESTON CAMPYLOBACTER SUPPLEMENT - Selective supplement for Campylobacter isolation
