TransScript Fly First-Strand cDNA Synthesis SuperMix
Provides high affinity to RNA template with fast extension rate
TransScript Fly First-Strand cDNA Synthesis SuperMix
Description
TransScript Fly RT is generated by site mutations. It provides high affinity to RNA template with fast extension rate.
The cDNA is efficiently synthesized by TransScript Fly RT/RI Enzyme Mix and 2×TS Fly Reaction Mix.
The entire reverse transcription can be completed within 5 minutes.
Highlights
- 5 minutes reverse transcription
- Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis
- Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA
- cDNA up to 12 kb
Applications
Multiple copy and low copy gene fast detection
Storage
At -20℃ for two year
Kit Contents
Component
|
TS/AF301/02
|
TS/AF301/03
|
TransScript® Fly RT/RI Enzyme Mix
|
50 µl
|
100 µl
|
2×TS Fly Reaction Mix
|
500 µl
|
1 ml
|
Random Primer(N9) (0.1 µg/µl)
|
50 µl
|
100 µl
|
Anchored Oligo(dT)18 Primer (0.5 µg/µl)
|
50 µl
|
100 µl
|
RNase-free Water
|
500 µl
|
1 ml
|