EasyScript First-Strand cDNA Synthesis SuperMix

Provides all the necessary components for cDNA synthesis from total RNA or mRNA

Scheda tecnica disponibile solo in lingua inglese

Description

EasyScript® First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by EasyScript® RT/RI Enzyme Mix and 2×ES Reaction Mix.

Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis
The product obtained from 15 minutes reaction is used for qPCR; the, product obtained from 30 minutes reaction is used for PCR
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA
cDNA up to 8 kb

Applications

Multiple copy gene detection

Storage

At -20℃ for two yearKit

Contents

Component	TS/AE301/02	TS/AE301/03
EasyScript RT/RI Enzyme Mix	50 µl	100 µl
2×ES Reaction Mix	500 µl	1 ml
Random Primer(N9) (0.1 µg/µl)	50 µl	100 µl
Anchored Oligo(dT)18 Primer (0.5 µg/µl)	50 µl	100 µl
RNase-free Water	500 µl	1 ml

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