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TransScript All-in-One First-Strand cDNA Synthesis SuperMix for PCR

Provides all the necessary components for cDNA synthesis from total RNA or mRNA

 Scheda tecnica disponibile solo in lingua inglese.

TransScript All-in-One First-Strand cDNA Synthesis SuperMix for PCR.

Description

TransScript All-in-One First-Strand cDNA Synthesis SuperMix for PCR provides all the necessary components for cDNA synthesis from total RNA or mRNA.

The SuperMix is provided at 5× concentration and used at 1× concentration by adding RNA and H2O.

The resulting cDNA are suitable for regular PCR, not for qPCR.

Highlights

  • One-tube format for simple and fast setup and reduce pipetting variability
  • The optimal ratio of oligo(dT)18 primer to random primer(N9) for PCR-ready cDNA
  • PCR-ready cDNA in 30 minutes (unsuitable for qPCR)
  • cDNA up to 12 kb

Applications

Multiple copy and low copy gene detection

Storage

At -20℃ for two year

Kit Contents

Component

TS/AT321/01
(50 rxns)

5×TransScript All-in-One SuperMix for PCR

200 μl

RNase-free Water

1 ml


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