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EasyScript Reverse Transcriptase
EasyScript Reverse Transcriptase is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity.
The enzyme is purified to near homogeneity from E.coli containing the modified M-MLV RT gene.
Applications
Unit Definition
One unit of EasyScript RT incorporates 1 nmol of deoxyribonucleotide into acid precipitable material in 10 minutes at 37℃ using Poly(A)/Oligo(dT) as template/primer.
5×ES RT Buffer
375 mM KCl, 15 mM MgCl2, 100 mM Tris-HCl pH 8.4
Storage
At -20℃ for two year
Kit contents
Component
TS/AE101/02
TS/AE101/03
EasyScript RT
10.000 U
5×10.000 U
200 μl
5×200 μl
Anchored Oligo(dT)18 Primer (0.5 µg/µl)
50 μl
250 μl