EasyScript Reverse Transcriptase

Engineered version of M-MLV reverse transcriptase with deficient RNase H activity

Scheda tecnica disponibile solo in lingua inglese

EasyScript Reverse Transcriptase is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity.

The enzyme is purified to near homogeneity from E.coli containing the modified M-MLV RT gene.

Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA
cDNA up to 8 kb

Applications

First-strand cDNA synthesis
Multiple copy gene detection

Unit Definition

One unit of EasyScript RT incorporates 1 nmol of deoxyribonucleotide into acid precipitable material in 10 minutes at 37℃ using Poly(A)/Oligo(dT) as template/primer.

5×ES RT Buffer

375 mM KCl, 15 mM MgCl2, 100 mM Tris-HCl pH 8.4

Storage

At -20℃ for two year

Kit contents

Component	TS/AE101/02	TS/AE101/03
EasyScript RT	10.000 U	5×10.000 U
5×ES RT Buffer	200 μl	5×200 μl
Anchored Oligo(dT)18 Primer (0.5 µg/µl)	50 μl	250 μl

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