TransFast Taq DNA Polymerase (with 2.5 mM dNTPs)
An engineered version of Taq DNA Polymerase
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TransFastTaq DNA Polymerase (with 2.5 mM dNTPs)
Description
TransFast Taq DNA Polymerase is an engineered version of Taq DNA Polymerase.
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa.
TransFast Taq DNA Polymerase has 5’-3’DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’exonuclease activity.
Highlights
- Extension rate is about 6 kb/min
- Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY-T vectors
- Amplification of genomic DNA fragment up to 4 kb
Applications
- Routine PCR
- High throughput PCR
- Colony PCR
Unit Definition
- One unit of TransFast Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃
Quality Control
TransFast Taq DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE.
Each batch of TransFast Taq DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.
Storage Buffer
- 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers
Storage
Kit Contents
|
Component
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TS/AP101/11
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TS/AP101/12
|
|
TransFast® Taq DNA Polymerase
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500 units
|
6×500 units
|
|
10× TransFast® Taq Buffer
|
1.2ml x 1
|
1.2ml x 6
|
|
2.5mM dNTPs
|
- / 800 μl x 1
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- / 800 μl x 6
|
|
6×DNA Loading Buffer
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1ml x 1
|
1ml x 6
|
