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TransFast Taq DNA Polymerase

The engineered version of Taq DNA Polymerase

Scheda tecnica disponibile solo in lingua inglese.

TransFastTaq DNA Polymerase

Description

TransFast Taq DNA Polymerase is an engineered version of Taq DNA Polymerase.

The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa.

TransFast Taq DNA Polymerase has 5’-3’DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’exonuclease activity.


Highlights

  • Extension rate is about 6 kb/min
  • Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY-T vectors
  • Amplification of genomic DNA fragment up to 4 kb


Applications

  • Routine PCR
  • High throughput PCR
  • Colony PCR


Unit Definition

  • One unit of TransFast Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃


Quality Control

TransFast Taq DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE.

Each batch of TransFast Taq DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.


Storage Buffer

  • 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers


Storage

  • At -20 ℃ for two years

Kit Contents

Component

TS/AP101/01

TS/AP101/02

TransFast® Taq DNA Polymerase

500 units

6×500 units

10× TransFast® Taq Buffer

1.2ml x 1

1.2ml x 6

6×DNA Loading Buffer

1ml x 1

1ml x 6


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