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TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix

Unique genomic DNA remover

 Scheda tecnica disponibile solo in lingua inglese.

TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix

Unique genomic DNA remover is combined with TransScript First-Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis.

After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds.


Highlights

  • Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination
  • The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR
  • cDNA up to 12 kb

Applications

Multiple copy and low copy gene detection

Storage

At -20℃ for two year

Kit Contents

Component

TS/AT311/02
(50 rxns)

TS/AT311/03
(100 rxns)

TransScript® RT/RI Enzyme Mix

50 μl

100 μl

gDNA Remover

50 μl

100 μl

2×TS Reaction Mix

500 μl

1 ml

Random Primer(N9) (0.1 μg/μl)

50 μl

100 μl

Anchored Oligo(dT)18 Primer (0.5 μg/μl)

50 μl

100 μl

RNase-free Water

500 μl

1 ml

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