TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix
Unique genomic DNA remover
Scheda tecnica disponibile solo in lingua inglese.
TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix
Unique genomic DNA remover is combined with TransScript First-Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis.
After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds.
Highlights
- Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination
- The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR
- cDNA up to 12 kb
Applications
Multiple copy and low copy gene detection
Storage
At -20℃ for two year
Kit Contents
|
Component
|
TS/AT311/02
(50 rxns)
|
TS/AT311/03
(100 rxns)
|
|
TransScript® RT/RI Enzyme Mix
|
50 μl
|
100 μl
|
|
gDNA Remover
|
50 μl
|
100 μl
|
|
2×TS Reaction Mix
|
500 μl
|
1 ml
|
|
Random Primer(N9) (0.1 μg/μl)
|
50 μl
|
100 μl
|
|
Anchored Oligo(dT)18 Primer (0.5 μg/μl)
|
50 μl
|
100 μl
|
|
RNase-free Water
|
500 μl
|
1 ml |
