TransStart KD Plus DNA Polymerase
Genetically modified high fidelity DNA polymerase
Scheda tecnica disponibile solo in lingua inglese.
TransStart KD Plus DNA Polymerase ( dNTPs-free)
Description
TransStart KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase.
This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3’-5’ exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
Highlights
• PCR products can be directly cloned into pEASY-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.
Applications
• Fast, high specificity amplification
• High fidelity, high yield amplification
Storage Buffer
50 mM Tris-Cl (pH 8.0)，50 mM KCl，1 mM DTT，0.1 mM EDTA，Stabilizers，50% glycerol.
Storage
At -20 ℃ for two years
Kit Contents
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Component
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TS/AP301/01
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TS/AP301/02
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TS/AP301/03
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TransStart KD Plus DNA Polymerase
|
100 U×1
|
200 U×1
|
200 U×6
|
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5×TransStart KD Plus Buffer
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1.2 ml×1
|
1.2 ml ×2
|
1.2 ml ×12
|
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50 mM MgSO4
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200 µl×1
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400 µl ×1
|
1 ml ×1
|
|
6×DNA Loading Buffer
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500 μl×1
|
1 ml ×1
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1 ml ×2
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