2×EasyPfu PCR SuperMix (-dye )
Ready-to-use mixture of EasyPfu DNA Polymerase, dNTPs and optimized buffer
2×EasyPfu PCR SuperMix (-dye )
Description
2×EasyPfu PCR SuperMix is a ready-to-use mixture of EasyPfu DNA Polymerase, dNTPs and optimized buffer.
The SuperMix is provided at 2× concentration and used at 1× concentration by adding template, primers and H2O.
- EasyPfu PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase
- Extension rate is about 0.5 kb/min
- PCR products can be directly cloned into pEASY®-Blunt vectors
- Amplification of genomic DNA fragment up to 6 kb
- Amplification of plasmid DNA fragment up to 10 kb
Applications
- High fidelity PCR
- Blunt end cloning
- Site-directed mutagenesis
Storage
At -20℃ for two years
Kit Contents
|
Component
|
TS/AS211/01
|
TS/AS211/02
|
|
2×EasyPfu PCR SuperMix (-dye)
|
1 ml
|
5×1 ml
|
|
ddH2O
|
1 ml
|
5 ml
|
Reaction System
|
Component
|
Volume
|
Final Concentration
|
|
Template
|
Variable
|
As required
|
|
Forward Primer (10 μM)
|
1 μl
|
0.2 μM
|
|
Reverse Primer (10 μM)
|
1 μl
|
0.2 μM
|
|
2×EasyPfu PCR SuperMix
|
25 μl
|
1×
|
|
ddH2O
|
Variable
|
-
|
|
Total volume
|
50 μl
|
-
|
