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2×EasyPfu PCR SuperMix (-dye )

Ready-to-use mixture of EasyPfu DNA Polymerase, dNTPs and optimized buffer

2×EasyPfu PCR SuperMix (-dye )

Description

2×EasyPfu PCR SuperMix is a ready-to-use mixture of EasyPfu DNA Polymerase, dNTPs and optimized buffer.

The SuperMix is provided at 2× concentration and used at 1× concentration by adding template, primers and H2O.

  • EasyPfu PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase
  • Extension rate is about 0.5 kb/min
  • PCR products can be directly cloned into pEASY®-Blunt vectors
  • Amplification of genomic DNA fragment up to 6 kb
  • Amplification of plasmid DNA fragment up to 10 kb


Applications

  • High fidelity PCR
  • Blunt end cloning
  • Site-directed mutagenesis


Storage

At -20℃ for two years

Kit Contents

Component

TS/AS211/01

TS/AS211/02

2×EasyPfu PCR SuperMix (-dye)

1 ml

5×1 ml

ddH2O

1 ml

5 ml

Reaction System

Component

Volume

Final Concentration

Template

Variable

As required

Forward Primer (10 μM)

1 μl

0.2 μM

Reverse Primer (10 μM)

1 μl

0.2 μM

2×EasyPfu PCR SuperMix

25 μl

ddH2O

Variable

-

Total volume

50 μl

-


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