2×EasyPfu PCR SuperMix (-dye )
Description
2×EasyPfu PCR SuperMix is a ready-to-use mixture of EasyPfu DNA Polymerase, dNTPs and optimized buffer.
The SuperMix is provided at 2× concentration and used at 1× concentration by adding template, primers and H2O.
Applications
Storage
At -20℃ for two years
Kit Contents
Component
TS/AS211/01
TS/AS211/02
2×EasyPfu PCR SuperMix (-dye)
1 ml
5×1 ml
ddH2O
5 ml
Reaction System
Volume
Final Concentration
Template
Variable
As required
Forward Primer (10 μM)
1 μl
0.2 μM
Reverse Primer (10 μM)
2×EasyPfu PCR SuperMix
25 μl
1×
-
Total volume
50 μl