2×TransTaq-T PCR SuperMix ( +dye)
Ready-to-use mixture of TransTaq-T DNA Polymerase, dNTPs and optimized buffer
2×TransTaq-T PCR SuperMix ( +dye)
Description
TransTaq-T PCR SuperMix is a ready-to-use mixture of TransTaq-T DNA Polymerase, dNTPs and optimized buffer.
The SuperMix is provided at 2× concentration and used at 1× concentration by adding template, primers and H2O.
Efficiency of PCR products with “A” is equal to EasyTaq DNA polymerase.
It is more suitable for high fidelity TA cloning.
• TransTaq-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq DNA Polymerase
• Extension rate is about 1-2 kb/min
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be cloned into pEASY-T vectors
• Amplification of genomic DNA fragment up to 8 kb
Applications
• Complex templates
• TA cloning
Storage
At -20℃ for two years
Kit Contents
|
Component
|
TS/AS122/11
|
TS/AS122/12
|
|
2×TransTaq-T PCR SuperMix(-dye)/(+dye)
|
1 ml
|
5×1 ml
|
|
ddH2O
|
1 ml
|
5 ml
|
Reaction System
|
Component
|
Volume
|
Final Concentration
|
|
Template
|
Variable
|
As required
|
|
Forward Primer (10 μM)
|
1 μl
|
0.2 μM
|
|
Reverse Primer (10 μM)
|
1 μl
|
0.2 μM
|
|
2×TransTaq-T PCR SuperMix
|
25 μl
|
1×
|
|
ddH2O
|
Variable
|
-
|
|
Total volume
|
50 μl
|
-
|